Feline herpesvirus 1 viral load related to environmental factors in sheltered cats

Feline herpesvirus 1 viral load related to environmental factors in sheltered cats

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ABSTRACT: Shelter environment stress factors are related to FHV-1 viral reactivation.However, comparisons between conjunctival viral LIPSTICK MAGENTA RUSH load and environmental factors have not been commonly evaluated.The aim of this study was to correlate FHV-1 viral load in domestic cats with and without clinical signs of conjunctivitis to shelter design in order to use FHV-1 viral load as a parameter of “health management”.Cats from four different shelters underwent an ophthalmological examination.Samples were collected by rolling a DNA/RNAse-free cytobrush over the ventral conjunctival fornix and were stored in 1.

5 mL sterile microtubes in 500 μL of Eagle’s minimum essential medium and kept at 4 ºC.Molecular procedures were performed up to 48 hours after collection.Different routines regarding new arrivals were directly related to FHV-1 viral load.Shelters where new arrivals occurred on daily basis had the highest viral load (2.69x108 copies/µL), while those shelters where new arrivals had not occurred in the few months prior to the beginning of the study had the lowest rate (1.

63x103 copies/µL).Environmental factors directly influenced FHV-1 DNA viral load.This study highlighted the need to improve the management approach in the animal shelter environment to reduce stressful situations responsible for Silicone Pad FHV-1 reactivation and higher viral load quantification.